Figure 7

Blockade of CSF-1R inhibits glioblastoma invasion in vivo. (A) C57Bl/6 mice were intracranially injected with GL261 cells and fed control (CTL) or PLX3397 chow as described in Figure 6. After paraffin embedding, sections were made and stained with Ki67, which was used to identify tumor cells. Representative images were taken with a 10× objective. Scale bar = 100 µm. (B) Invasion in tumors isolated from animals fed with control chow (open bar) or PLX3397 chow (closed bar) was quantified by counting the number of tumor cells that were beyond the border and normalized to the length of the border (in µm). Results shown are means ± SEM of data of six tumors. *P < 0.05 compared with control.