Figure 4

Induction of the decreased ING4 expression by DTX in parental SPC-A1 cells. (A) SPC-A1 cells were cultured in the presence of various concentrations of DTX (0, 1, 2, 3, 4 or 5 µg/L) for 24 h. Real-time quantitative RT-PCR assay was performed to detect the expression of ING4 mRNA. *p < 0.05 compared with 0 µg/L. (B) SPC-A1 cells were cultured in the presence of DTX (1.0 µg/L) for various time (0, 1, 3, 5, 7 or 14 d). Real-time quantitative RT-PCR was performed to detect the expression of ING4 mRNA. Results represent the average of three independent experiments (mean ± SD). *p < 0.05 and **p < 0.01 compared with 0 d.