Figure 5
Effect of ING4 overexpression on the in vitro sensitivity of SPC-A1/DTX or A549/ Taxol cells to DTX or paclitaxel. (A) Real-time quantitative RT-PCR analysis of ING4 mRNA expression in the stably transfected SPC-A1/DTX/ING4 (or SPC-A1/DTX/control or parental SPC-A1) and A549/Taxol/ING4 cells (or A549/Taxol or parental A549). (B) Western blot analysis of ING4 protein expression in the stably transfected SPC-A1/DTX/ING4 (or SPC-A1/ DTX/control or parental SPC-A1) and A549/Taxol/ING4 cells (or A549/Taxol or parental A549). (C) MTT analysis of the IC50 values of DTX or paclitaxel in SPC-A1/DTX/ING4 (or SPC-A1/DTX/control) and A549/Taxol/ING4 (or A549/Taxol/control) cells. Results represent the average of three independent experiments (mean ± SD). *p < 0.05 and **p < 0.01 compared with SPC-A1/DTX/control or A549/Taxol/control cell line.