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Figure 2 | Molecular Medicine

Figure 2

From: c-Met Inhibitor Synergizes with Tumor Necrosis Factor-Related Apoptosis-Induced Ligand to Induce Papillary Thyroid Carcinoma Cell Death

Figure 2

Effect of PHA665752 treatment on c-Met and its downstream signaling pathway (A) B-CPAP and TPC-1 cells were treated with various doses of PHA665752 as indicated and cytoplasmic extracts were prepared; 10 µg of protein from each sample was separated on SDS-PAGE, transferred to a PVDF membrane and immunoblotted with antibodies against total Met, p-Met, p-AKT, Foxo1, p-GSK3 and p-Bad. The blots were probed with an antibody against β-actin for equal loading. (B) B-CPAP and TPC-1 cells were transfected with scrambled siRNA (100 nmol/L) and c-Met siRNA (50 and 100 nmol/L) for 48 h. After 48 h, cells were lysed and proteins were immunoblotted with antibodies against c-Met, p-Met, AKT, p-AKT, caspase-3 and β-actin.

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Molecular Medicine

ISSN: 1528-3658