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Figure 5 | Molecular Medicine

Figure 5

From: Glycine N-Methyltransferase Deficiency Affects Niemann-Pick Type C2 Protein Stability and Regulates Hepatic Cholesterol Homeostasis

Figure 5

GNMT stabilizes NPC2 protein. (A) Analysis of NPC2 degradation followed by cycloheximide (CHX) treatment. The 293T cells were transfected with either NPC2-HA or GNMT-Flag plus NPC2-HA for 24 h before treatment with CHX for the indicated hours. NPC2 degradation was analyzed using Western blot. Single arrowheads indicate mono-glycosylated NPC2; double arrowheads indicate diglycosylated NPC2. (B) Pulse-chase analysis of NPC2. The 293T cells were transfected with either NPC2-HA or GNMT-Flag plus NPC2-HA for 24 h; 35S-Met/Cys incorporation measurements were used to determine NPC2 half-lives. −□−, pNPC2; −■−, pNPC2 + pGNMT.

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Molecular Medicine

ISSN: 1528-3658