Figure 7

NPC2 is dysregulated in fatty liver tissues. (A) IHC staining of human normal and fatty liver using a monoclonal NPC2 antibody. Original magnification 200×. Western blot analysis of NPC2 expression from WT mice fed with high-cholesterol diet (B), high-fat diet (C) or MCD diet (E). (D) 8-wk-old ob/ob mice (n = 3–5 per group). (F) For endoglycanase digestion, liver lysates (60 µg) from 0- and 5-wk MCD diet-fed mice were incubated with PNGase F and subjected to Western blot analysis.