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Figure 3 | Molecular Medicine

Figure 3

From: Serum from Patients Undergoing Remote Ischemic Preconditioning Protects Cultured Human Intestinal Cells from Hypoxia-Induced Damage: Involvement of Matrixmetalloproteinase-2 and -9

Figure 3

Sera derived after RIPC contain reduced enzymatic activities of MMP-2 and MMP-9. (A) SDS-PAGE/silver staining of serum proteins and gelatin zymography for the detection of enzymatically active MMPs. Activities of MMP-2 and MMP-9 are significantly reduced in protective sera T2 compared with nonprotective sera T0. (B) Densitometric evaluation of MMP-9 and MMP-2 enzymatic activities. Numbers in the columns display the numbers of sera used in the respective experiment. Columns show the mean ± SEM. *P < 0.05, two-tailed t test; **P < 0.01, two-tailed ttest. rdu, relative densitometric units.

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