Figure 1

BIN1 mRNA and protein expression were remarkably reduced in human HCC cell lines and in primary HCC surgical specimens assessed with real-time quantitative PCR and Western blot. (A, B) BIN1 mRNA expression in human HCC cell lines. The normal liver cell line LO2 was used as a positive control. The BIN1 mRNA expression level was normalized to GAPDH and LO2 or to β-actin and LO2. Experiments were performed four times (*P< 0.05 compared with LO2). (C) BIN1 protein expression in human HCC cell lines. (D, E) Density of BIN1 bands normalized to GAPDH and LO2 or to β-actin and LO2. Experiments were performed in three times (*P < 0.05 compared with LO2). (F) BIN1 mRNA expression in human primary HCC tumor tissues and adjacent nontumor liver tissues (n = 42). The BIN1 mRNA expression level was normalized to GAPDH. Experiments were performed four times. The difference in the expression levels was analyzed with the paired t test (P = 0.02). (G) BIN1 protein expression in six representative human primary HCC tumor tissues (T) and adjacent nontumor liver tissues (N). (H) Density of BIN1 bands normalized to GAPDH. Experiments were performed three times. *P< 0.05 compared T (tumor tissue) versus N (adjacent nontumor liver tissue). H: ■, N; □, T.