Figure 5

Enforced expression of BIN1 inhibited HCC cell migration and metastatic capability. (A) The migration ability of BIN1 vector- or control vector-transfected HCC cells was determined by wound-healing assay. The ability of Hep3B and Huh7 cells grown in a confluent monolayer to migrate into a linear wound created by a pipette tip was monitored for 24 h. BIN1 overexpression was confirmed by immunoblotting and the density of each band was quantitated. (B) Cell invasion of HCC cells transfected with BIN1 or control vector was evaluated with Matrigel assay. Invasive cells were stained, photographed and counted under the microscope. Right, quantitative analyses of stained cell numbers are shown as values of mean ± standard deviation. Experiments were performed in triplicate. P values were calculated using the Student t test.