Figure 6

Analysis of proliferation, migration and invasion of HCC cell lines compared with normal liver cell line. (A) Proliferation detection in HCC cell lines and normal liver cell line. The growth rate of HCC cell lines with overexpressed BIN1 was lower than the control but was similar to the normal liver cell line LO2 (*P < 0.05). (B) The migration ability of normal liver cells and BIN1 vector- or control vector-transfected HCC cells was determined by wound-healing assay. The migration ability of HCC cells with overexpressed BIN1 was lower than control and was similar to LO2. (C) Cell invasion of normal liver cells and HCC cells transfected with BIN1 or control vector was evaluated with Matrigel assay. The invasion ability of HCC cells overexpressing BIN1 was lower than control. LO2 did not show invasion. Experiments were performed in triplicate. A: ●, BIN1; ■, vector; ▲, LO2.