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Figure 1 | Molecular Medicine

Figure 1

From: Benefits of Recombinant Adeno-Associated Virus (rAAV)-Mediated Insulinlike Growth Factor I (IGF-I) Overexpression for the Long-Term Reconstruction of Human Osteoarthritic Cartilage by Modulation of the IGF-I Axis

Figure 1

Detection of transgene expression in monolayer and alginate cultures of rAAV-transduced human articular chondrocytes and human ACL fibroblasts. Primary human normal and OA chondrocytes were transduced with either rAAV-hIGF-I or a control vector (rAAV-RFP or rAAV-lacZ) for maintenance in monolayer (A, C, E: MOI = 20) or alginate culture (D: MOI = 6). The vectors were also applied to monolayer cultures of primary human ACL fibroblasts using increasing vector doses (B: MOI = 20, 200 or 2,000). Transgene expression was detected by live fluorescence (A: magnification 10×; B: magnification 20×; insets: same fields under transmitted light; d 20 after transduction), anti-IGF-I immunocyto- and immunohistochemistry (C: d 20 after transduction; D: d 26 after transduction; both: magnification 20×) and Western blotting analysis (E: lane 1, rAAV-lacZ-transduced OA chondrocytes; lane 2, rAAV-hIGF-I-transduced OA chondrocytes; d 5 after transduction).

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Molecular Medicine

ISSN: 1528-3658