Skip to main content
Figure 2 | Molecular Medicine

Figure 2

From: Benefits of Recombinant Adeno-Associated Virus (rAAV)-Mediated Insulinlike Growth Factor I (IGF-I) Overexpression for the Long-Term Reconstruction of Human Osteoarthritic Cartilage by Modulation of the IGF-I Axis

Figure 2

Analysis of cell proliferation and extracellular matrix synthesis in rAAV-transduced human articular chondrocytes in monolayer and alginate cultures. Primary human normal and OA chondrocytes were transduced with either rAAV-lacZ or rAAV-hIGF-I, as described in Figure 1, and cell encapsulation was performed where indicated, as also denoted in Figure 1. Monolayer cultures were processed for the evaluations on d 20 after transduction and alginate cultures on d 26. (A, B) BrdU immunodetection in monolayer (A: magnification 10×) and alginate (B: magnification 20×) cultures of transduced chondrocytes after BrdU incorporation. (C) H&E staining of histological sections from transduced alginate cultures (magnification 20×). (D) Immunodetection of type II collagen on sections from transduced alginate cultures (magnification 20×).

Back to article page

Molecular Medicine

ISSN: 1528-3658