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Figure 3 | Molecular Medicine

Figure 3

From: Benefits of Recombinant Adeno-Associated Virus (rAAV)-Mediated Insulinlike Growth Factor I (IGF-I) Overexpression for the Long-Term Reconstruction of Human Osteoarthritic Cartilage by Modulation of the IGF-I Axis

Figure 3

Immunohistochemical analyses in rAAV-transduced human articular cartilage. Primary human normal and OA cartilage explant cultures were transduced with either rAAV-hIGF-I or the control rAAV-lacZ vector (4 × 108 each functional vector) and processed after 90 d to detect transgene expression by anti-IGF-I immunohistochemistry (A: magnification 20×), the expression of major extracellular matrix components (B: safranin O at magnification 10× and type II collagen in the insets at magnification 10×), the rates of cell proliferation after BrdU incorporation and immunodetection (C: magnification 20×) and apoptotic events by TUNEL assay (D: magnification 4×). All are views of the middle zone.

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Molecular Medicine

ISSN: 1528-3658