Table 2 Biochemical analyses in rAAV-transduced alginate-chondrocyte spheres.

Cells (10⁴/sphere)

5

1.40 (0.02)

1.70 (0.03)^a

1.30 (0.07)

1.80 (0.10)^a

26

0.04 (0.02)^b

1.55 (0.05)^a

0.04 (0.05)^b

1.45 (0.04)^a

Cell densities (cells/mm²)

5

122 (5)

178 (9)^a

119 (4)

165 (3)^a

26

16 (1)^b

149 (7)^a

14 (2)^b

137 (6)^a

DNA (µg/10⁴ cells)

5

0.36 (0.03)

3.07 (0.14)^a

0.32 (0.02)

3.18 (0.16)^a

26

0.12 (0.01)^b

2.88 (0.12)^a

0.10 (0.01)^b

2.73 (0.11)^a

Proteoglycans (µg/10⁴ cells)

5

3.91 (0.16)

4.70 (0.25)^a

3.36 (0.14)

4.42 (0.21)^a

26

3.07 (0.12)^b

10.91 (1.04)^a,b

2.97 (0.11)^b

9.37 (1.01)^a,b

Type II collagen (ng/10⁴ cells)

5

0.14 (0.03)

0.33 (0.04)^a

0.09 (0.01)

0.28 (0.04)^a

26

0.12 (0.02)^b

0.79 (0.05)^a,b

0.08 (0.01)^b

0.54 (0.05)^a,b

Type II collagen staining (%)

5

18 (3)

37 (5)^a

15 (5)

29 (4)^a

26

15 (3)^b

70 (4)^a,b

12 (3)^b

65 (3)^a,b