Expression of GNMT is elevated in the aortas of apoE−/− mice. A) Aortic specimens from WT and 5-month-old apoE−/− mice were immunostained with anti-GNMT, anti-vWF or anti-F4/80 antibody and then recognized by the corresponding horseradish peroxidase-conjugated secondary antibody. Antigenic sites were visualized by the addition of DAB. Hematoxylin was used for counterstaining. The vWF-positive cells and F4/80-positive cells (brown color) denoted endothelial cells and macrophages, respectively. Magnification: 100×. B) Western blot analysis of protein expression of GNMT in aortic lysates from WT and apoE−/−. α-Tubulin was a loading control. C, Western blot analysis of GNMT protein expression in BMDMs treated with 50 µg/mL oxLDL for the indicated times. Data are mean ± SEM from five independent experiments. *P < 0.05 versus WT mice or vehicle-treated group.