Deletion of GNMT in apoE−/− mice impairs cholesterol metabolism in aortas and liver. A, The capacity of reverse cholesterol efflux of WT, GNMT−/−, apoE−/− or apoE−/− GNMT−/− mice. B) Western blot analysis and quantitation of protein levels of SR-A, CD36, SR-BI, ABCA1 and ABCG1 in aortas of apoE−/− and apoE−/− GNMT−/− mice. C) The hepatic concentrations of cholesterol and triglyceride were assessed by colorimetric assay kits. D) Western blot analysis and quantitation of protein expression of SR-BI, ABCG1, ABCG5, ABCG8, ABCA1 in mouse liver. α-Tubulin was a loading control. Data are mean ± SEM from 14 mice. *P < 0.05 versus WT mice or apoE−/− mice, #P < 0.05 versus GNMT−/− mice, &P < 0.05 versus apoE−/− mice.