Figure 6

Loss of GNMT impairs RCT-dependent cholesterol efflux in macrophages. A) For Dil-oxLDL binding assay, BMDMs from WT and GNMT^−/− mice were incubated with Dil-labeled oxLDL at 4°C for 4 h, then fluorescence was determined. For cholesterol efflux assay, BMDMs from WT and GNMT^−/− mice were treated with or without oxLDL (50 µg/mL) for 12 h and then with NBD-cholesterol for another 6 h. Cholesterol efflux was defined as the fluorescence in medium from each group. B) BMDMs were treated with or without oxLDL (50 µg/mL) for 24 h. Western blot analysis and quantitation of the protein expression of SR-A, CD36, SR-BI, ABCA1, and ABCG1. α-Tubulin was a loading control. Data are mean ± SEM from five independent experiments. *P < 0.05 versus WT nontreated group, ^#P < 0.05 versus WT oxLDL-treated group.