Figure 5

Blocking necroptosis deteriorates acute experimental pancreatitis. Cerulein-induced pancreatitis mice were left untreated or given i.p. injections of cerulein once every hour for 10 h. In addition to every second injection of cerulein, either vehicle or zVAD, Nec-1 and TAT-crmA were injected as indicated. 14 h after the last injection mice were sacrificed and blood samples were taken. Levels of serum amylase (A) and serum lipase (B) are shown for each group as indicated. In parallel, pancreatic tissue samples were harvested. Representative sections are demonstrated in C, and a pancreatic damage score was used for quantification (D) (n = 5, * P < 0.05, ** P < 0.02). Scale bar, 50 µm.