Figure 3

rIL-33 treatment induces Th2 cytokines and inhibits Th1 cytokines in mice with TNBS-induced colitis. Mice instilled with TNBS were injected intraperitoneally with rIL-33 or PBS daily. The serum, MLN cells and LPMCs were collected from mice killed d 4 after TNBS inoculation. (A) Cytokines (IL-4, IL-5, IL-13, IFN-γ and IL-17) in sera were determined by ELISA (pg/mL). (B) MLN cells were stimulated with α-CD3/α-CD28 and the cultural supernatants were harvested, followed by ELISA analysis of cytokines indicated above (ng/mL). (C) Total mRNA was extracted from colonic tissues to analyze the expression of IL-4, IL-5, IL-13, IFN-γ, IL-17, IL-6, IL-10 and TNF-α by real-time PCR. The data are presented as mean ± SD (n = 6-8/group). (D) MLN cells and LPMCs were obtained at indicated time points and pooled; membrane expression of ST2L in the CD4⁺ subset was detected by flow cytometry. Data are shown as the mean ± SD (n = 6-8/group) and are representative of three independent experiments; *p < 0.05, ^#p < 0.01.