Figure 4

rIL-33 treatment increases the numbers in the CD103⁺CD11c⁺ tolerogenic DC sub-population. MLN cells and LPMCs were isolated from the colitis mice treated with rIL-33 or PBS on d 4 after administration of TNBS (A–C). MLN cells (A) and LPMCs (B) were analyzed by flow cytometry for CD103⁺CD11c⁺ DCs (C), and data are presented as the percentage of CD103⁺CD11c⁺ cells in total MLN cells (upper panel) or CD11c⁺ subset (lower panel). Data represent mean ± SD per group (n = 6-8/group). The results shown are from one of three independent experiments. (D) MLN cells from normal mice were collected to analyze the CCR7 and IDO expression in subpopulation by CD103. The mean fluorescence intensity (MFI) of IDO expression in the two subpopulations was recorded by flow cytometry. The data are presented as mean ± SD of four mice investigated; *p < 0.05, ^#p < 0.01.