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Figure 1 | Molecular Medicine

Figure 1

From: Expression of Interleukin-8 Receptor CXCR2 and Suppressor of Cytokine Signaling-3 in Astrocytic Tumors

Figure 1

CXCR2, SOCS-3 and STAT-3 protein levels in fresh-frozen tissue specimens by Western blot and immunohistochemical expression of CXCR2, SOCS-3 and IL-8 in formalin-fixed tissue from the same six specimens. (A) Detection of CXCR2 (Western blot and immunohistochemistry) in the six cases. Tumors 4 and 5 showed the highest CXCR2 levels in frozen and paraffin-embedded samples. (B) Detection of SOCS-3 (Western blot and immunohistochemistry) in the six cases. Tumors 1, 3 and 5 showed the highest SOCS-3 levels in frozen and paraffin-embedded samples. (C) Detection of p-STAT-3 by Western blot in the six cases. (D) Immunohistochemical detection of IL-8 in the six cases. (E) Actin used as an internal control for Western blot analysis in the six cases. Negative controls indicate cases in which CXCR2, SOCS-3 and IL-8 have been substituted by nonimmune serum. N, area at necrosis; V, blood vessel.

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Molecular Medicine

ISSN: 1528-3658