Figure 6

Enhanced TNF-α and CXCL1 release by IRAK-M^−/− macrophages. TNF-α, IL-6, CXCL1 and CXCL2 concentrations in (A-D) alveolar macrophage supernatants from WT (black bars) and IRAK-M^−/− (white bars) mice after ex vivo incubation with RPMI, LPS from K. pneumoniae (LPS KP 10 µg/mL) or growth-arrested K. pneumoniae (GAKP MOI 1:100) for 16 h (n = 4/group, *P < 0.05 as compared with medium stimulation), (E-H) bronchoalveolar lavage fluid and (I-L) lung homogenates from WT (black bars/closed symbols) and IRAK-M^−/− (white bars/open symbols) mice 3–48 h after infection with 1 × 10⁴ CFU of K. pneumoniae. Data are expressed as mean ± SEM; n = 7–9/group, *P < 0.05, **P < 0.01 as compared with WT mice.