Effect of MSC administration on survival and disease progression of SOD1/G93A mice. Animals were intravenously injected with 106 MSCs or saline at d 90 and monitored for motor abilities and body weight. Survival time was determined when animals were unable to roll over within 20 s after being pushed on their side. Eleven MSC-treated and 13 saline-treated SOD1/G93A mice were used. (A) Survival. Survival time was examined by Kaplan-Meyer analysis. The difference between Kaplan-Meyer curves was significant at p < 0.01 (log-rank test). (B) Rotarod. Animals were tested starting on d 60 every 5 d until d 9 and were then examined 3 d/wk. Falling off time was recorded. (C) Extension reflex. Measures were obtained as reported in (A). Animals were rated according to a 5-0 score scale (see Supplementary Table S1). (D) Gait impairment. Measures were obtained as reported in (A). Each animal was rated according to a 5-0 score scale (see Supplementary Table S1). (E) Body weight. Body weight was measured immediately before behavioral testing. Values were compared with those from saline-injected SOD1 mice (n = 12). Means ± SEM are reported. *p < 0.05 at least (Mann-Whitney).