Figure 3

Binding of histone H3 to macrophages is diminished by the opsonins Gas6 and MFG-E8. Macrophages were incubated with BSA (control), Gas6 or MFG-E8 (5 μ03g/mL) for 1 h and then washed three times, followed by incubation with Chromeo 488-conjugated histone H3 (H3-488) or Chromeo 488-conjugated BSA (BSA-488) (5 μg/mL) for 1 h. The cells were then washed again with PBS to remove unbound proteins. The quantities of proteins bound to macrophages were determined by a fluorescent plate reader. ⋆p< 0.05 and ⋆⋆⋆p < 0.001 compared with the control group.