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Figure 8 | Molecular Medicine

Figure 8

From: Pigment Epithelium-Derived Factor (PEDF) Peptide Eye Drops Reduce Inflammation, Cell Death and Vascular Leakage in Diabetic Retinopathy in Ins2Akita Mice

Figure 8

Regulation of ZO1 and occludin expression by the PEDF derivatives through the PI3K/AKT pathway. Quantitative PCR measurements indicate that the effects of P60 and P78 on ZO1 and occludin expression are mediated through the PI3K/AKT pathway in a retinal explant model of diabetes. Explants were cultured in high glucose (25 mmol/L) for 24 h in the presence/absence of the pharmacological inhibitors of PI3K (iPI3K; LY294,002), MAPK (iMAPK; U0126) and one or the other of the PEDF derivatives (n = 4). iPI3K blocked P78 effects on both ZO1 and occludin expression. It also inhibited the effects of P60 on ZO1 expression but not on occludin levels. p values are indicated for statistically significant comparisons. All values are means ± SD.

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Molecular Medicine

ISSN: 1528-3658