Figure 6
From: MGMT Inhibition Restores ERα Functional Sensitivity to Antiestrogen Therapy
Histology and IHC analysis. Mice with mammary tumors were treated with BG or tamoxifen or ICI, or combination therapy (BG + TAM/ICI). The sections were immunostained for expression of MGMT, ERα, p53, p21 and ki-67. Tumors from mice treated with BG or combination therapy had a significant decrease in the expression of MGMT, ERα and ki-67. p53 expression was not altered significantly in these treatment groups. Expression of p21 was increased significantly in all treatment groups compared with controls. Representative samples (40×) are shown. IHC correlative quantified expression analysis was done using ImageJ/ImmunoRatio plugin, as described in the Materials and Methods.