| | Breast Tumors |
|---|
| | | Tumor weight (mg) | Tumor volume (mm3) | Body weight (g) |
Treatment groupa | Tumor incidence | Mean ± SD | Range | Mean ± SD | Range | Mean ± SD | Range |
Control | 10/10 | 260.6 ± 18.3 | 237.0&−294.0 | 254.8 ± 28.7 | 223.2−316.5 | 26.31 ± 1 | 25.2−27.4 |
TAM | 10/10 | 257.0 ± 20.5 | 234.6−301.2 | 270.6 ± 25.5 | 240.6−331.5 | 26.02 ± 1.23 | 23.2−27.2 |
ICI | 10/10 | 248.0 ± 18.9 | 227.7−289.9 | 241.5 ± 17.2 | 220.8−270.3 | 25.74 ± 1.25 | 23.4−27.3 |
BG | 10/10 | 94.2b ± 33.3 | 30.3−144.6 | 120.2c± 27.1 | 76.2−175.8 | 25.31 ± 1 | 23.9−26.8 |
TAM + BG | 10/10 | 32.5d± 8.9 | 18.3−45.6 | 62.0e ± 21.0 | 19.2−96.6 | 25.21 ± 0.7 | 24.2−26.4 |
ICI + BG | 10/10 | 58.2f ± 16.9 | 31.8−78.9 | 75.1g± 14.4 | 46.8−97.5 | 25.69 ± 1 | 24.3−26.8 |
- Tamoxifen-resistant breast cancer cells (7 × 106) were injected into the breast of nude mice. Groups of mice were randomized 10 d later and treated with twice weekly (Tuesday, Thursday) IP injections of tamoxifen or ICI (1 mg/kg), versus daily (Monday through Friday (M-F)) IP injections of BG (10 mg/kg) alone versus combination therapy which received twice weekly IP injections of tamoxifen or ICI and daily BG (M-F) versus control group which received daily (M-F) 1 × PBS. All mice were euthanized on d 42. Tumor size and volume in mice treated with BG alone and in combination with TAM/ICI were significantly smaller when compared with mice treated with TAM/ICI alone or to the control group.
- aMCF7-luc human breast cancer cells (7 × 106) were injected into the mammary fat pad of female ovarectomized nude mice. After a palpable tumor was formed, six groups of mice were treated: vehicle solution; TAM (1 mg/kg); ICI (1 mg/kg); BG (10 mg/kg); TAM + BG and ICI + BG. All mice were euthanized on d 42.
- bP < 0.0001 (BG) compared with control and P < 0.0001 (BG) compared with TAM + BG (d) and ICI + BG (f).
- cP < 0.0001 (BG) compared with control and P < 0.0001 (BG) compared with TAM + BG (g) and ICI + BG (e). dP < 0.0001 (TAM + BG) compared with control and P < 0.0001 (TAM + BG) compared with TAM alone.
- eP < 0.0001 (TAM + BG) compared with control and P < 0.0001 (TAM + BG) compared with TAM alone.
- fP < 0.0001 (ICI + BG) compared with control and P < 0.0001 (ICI + BG) compared with ICI alone.
- gP < 0.0001 (ICI + BG) compared with control and P < 0.0001 (ICI + BG) compared with ICI alone;.
