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Figure 4 | Molecular Medicine

Figure 4

From: The Infective Polymerization of Conformationally Unstable Antithrombin Mutants May Play a Role in the Clinical Severity of Antithrombin Deficiency

Figure 4

Antithrombin secretion and retention under stress conditions. HEK-EBNA cells were transfected with 200 ng of WT/WT plasmid or cotransfected with 100 ng of WT plasmid and 100 ng of Δ393 plasmid (WT/London) and then treated at different pHs (A) and temperatures (B). Culture medium from cells were collected after treatment and analyzed by SDS-PAGE under reduciμg conditions by western blot with an antibody to antithrombin. Intracellular antithrombin was detected by immunofluorescence.

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Molecular Medicine

ISSN: 1528-3658