Figure 3

Inhibitory effect of ICD on xenografts formed by SP and non-SP cells from the MHCC-97L line. (A) Tumor masses formed by MHCC-97L-SP or non-SP cells and treated with ICD or PBS in nude mice. (B) The weights of tumor masses induced by MHCC-97L SP or non-SP cells and treated with ICD or PBS (n = 5 each group, *p < 0.05). (C) The weights of tumor-bearing mice treated with ICD or PBS. (D) TUNEL assay of apoptotic cells in SP and non-SP cells induced subcutaneous tumor tissues of control groups (PBS) and ICD treatment groups. (E) Liver orthotopic SP and non-SP cells induced tumors treated with PBS or ICD. (F) TUNEL assay of apoptotic cells in SP and non-SP cells induced liver orthotopic tumor tissues of control groups (PBS) and ICD treatment groups. (G) Western blot of caspase proteins from the tumor tissues of the four groups. (H) Western blot of apoptosis-related proteins from the tumor tissues of the four subcutaneous xenograft groups.