Figure 4

ICD increases the sensitivity of HCC cell lines to DXR. (A) Cell cytotoxity detection with MTT assay; pretreatment with 150 µg/mL ICD increased the sensitivity of MHCC-97L and MHCC-LM3 cells to DXR treatment, and pretreatment with 10 µg/mL DXR decreased their sensitivity to DXR. (B) DXR efflux and retention assays showed that pretreatment with ICD reduces DXR efflux and prolongs its retention in both MHCC-97L and MHCC-LM3 cells. The left panel (red) shows the autofluorescence of DXR, the middle panel is the bright field image and the right panel is the merge of the previous two images. *p < 0.05; **p < 0.01.