Figure 5

PDCD4 is a potential target of ICD. (A) Western blot of ABCG2 and PDCD4 in sorted SP and non-SP cells from the MHCC-97L cell line. (B) Western blot of PDCD4 in MHCC-97L and MHCC-LM3 cells exposed to ICD for 24 h. (C) Western blot of PDCD4 in sorted SP and non-SP cells from MHCC-97L and MHCC-LM3 lines treated with PBS or 150 µg/mL ICD for 24 h. (D) Western blot of PDCD4 in tumors induced by MHCC-97L SP or non-SP cells and treated with ICD or PBS, respectively. (E) Western blot of PDCD4 protein in MHCC-97L cells after RNA interference. NC, negative control. (F) Analysis of ICD-induced apoptosis in SP and non-SP cells after PDCD4 siRNA. After silencing of PDCD4, cells were treated with 150 µg/mL ICD for 48 h.