Figure 2

Influence of IL-1β on osteoblast migration toward several growth factors with IL-1β present in a uniform concentration (A) or as a gradient (B). In vitro migration was analyzed in a modified Boyden chamber using 8 µm polycarbonate filters. IL-1β was diluted in serum-free DMEM 100 pg/mL (black bars) and added either to both upper and lower (A) or only to lower (B) wells of the Boyden chamber as indicated. Serum-free DMEM was used as control (white bars). Growth factors were diluted in serum-free medium at the following concentrations: PDGF-BB (10 ng/mL), IGF-1 (100 ng/mL) and VEGF-A (100 ng/mL). Columns represent mean number of totally migrated cells, bars represent SEM from three independent donors. Significance was calculated with two-way ANOVA followed by a Bonferroni posthoc test; *P <: 0.05. (A) Migration of osteoblasts toward growth factors with IL-1β in upper and lower wells. (B) Migration of osteoblasts toward growth factors with IL-1β only in lower wells.