Figure 3

Influence of IL-1β on osteoblast adhesion. In vitro adhesion assay was performed in a modified Boyden chamber using 8 µm polycarbonate filters. IL-1β was diluted in serum-free DMEM at 100 pg/mL and added either to both upper and lower (gray bars) or only to lower wells (black bars) of the Boyden chamber as indicated. Serum-free DMEM was used as negative control (white bars). Columns represent mean number of adhered cells from one donor.