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Figure 2 | Molecular Medicine

Figure 2

From: miR-133a Regulates Vitamin K 2,3-Epoxide Reductase Complex Subunit 1 (VKORC1), a Key Protein in the Vitamin K Cycle

Figure 2

Validation and specificity of VKORC1 mRNA-miRNA interaction in HepG2 cells. (A) HepG2 cells were transfected with precursor molecules for miR-133a and with an SCR at three different concentrations. (B) HepG2 cells were transfected with 200 nmol/L of precursors for miR-133a and with an SCR control and tested for F8 and SERPINC1 mRNA expression by qRT-PCR. Results are represented as means ± standard deviation (SD) from five experiments performed in triplicate. Student ttest was calculated in SCR versus pre-miR (*P< 0.05; **P< 0.01; ***P< 0.001). (C) Expression of endogenous VKORC1 in HepG2 after transfection with miR-133a (100 nmol/L). β-actin serves as the loading control.

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Molecular Medicine

ISSN: 1528-3658