miR-133a Regulates Vitamin K 2,3-Epoxide Reductase Complex Subunit 1 (VKORC1), a Key Protein in the Vitamin K Cycle

Table 1 Specific scores, free energy values and characteristics of the VKORC1 3′UTR/miRNAs binding.

MicroRNA (hsa-miR)	Context score percentile	Conserved branch length score	p ^Ct	Start site	End site	Binding free energy (kcal/mol)
Conserved sites for miRNA families
133 p ]137	94 p ]86	1.659 p ]1.937	0.77 p ]0.87	145 p ]254	151 260	−18.7 p ]−19.3
Poorly conserved sites and sites for poorly conserved miRNA families
147b	75	0.01	NA	131	137	−19.5
330-3p	50	1.396	NA	30	36	−17.6
330-3p	56	1.396	NA	35	41	−17.6
1296	80	0.517	NA	85	91	−14
765	44	0.01	NA	116	122	−9.3
644	76	0.396	NA	156	162	−11.4
1276	75	0.01	NA	167	176	−8.6
1276	90	0.01	NA	221	226	−8.6
612	85	0.319	NA	232	238	−11.5
1285	85	0.319	NA	232	238	−22
1207-5p	42	0.323	<0.1	123	127	−9.2
183	7	0.323	NA	123	129	−10.8
609	37	0.01	NA	177	183	−10.2
620	28	0.048	NA	213	219	−8.1
1270	17	0.448	NA	213	219	−19.7
642	48	1.282	NA	240	246	−20.4
1178	84	1.207	NA	252	258	−9.3

Details of these scores can be found at https://doi.org/www.targetscan.org. Binding free energy values were calculated by using the RNAHybrid algorithm (see Materials and Methods). NA, not applicable.

ISSN: 1528-3658