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Figure 3 | Molecular Medicine

Figure 3

From: p38 Mitogen-Activated Protein Kinase/Signal Transducer and Activator of Transcription-3 Pathway Signaling Regulates Expression of Inhibitory Molecules in T Cells Activated by HIV-1-Exposed Dendritic Cells

Figure 3

HIV-1 induces STAT3 activation and impairs T-cell functionality. Matured DCs were pulsed with mock or HIV-1BaL (HIV), cultured for ∼24 h, washed and cocultured with TN cells for 8 d. (A) Intracellular staining for phosphorylated STAT3 was performed after 8 d to compare between HIV-1 primed and mock DC primed T cells. MERK-573095 (200 µmol/L) or Sigma WP1066 (2 µmol/L) STAT3 inhibitors were added to the wells at the onset of cocultures. (B) T-cell proliferation was measured by CFSE dilution by flow cytometry and 3H-thymidine incorporation (n = 6). (C) The expression levels of CTLA-4, PD-1, TRAIL, TIM-3, CD160, LAG3, FOXP3, BLIMP-1 and DTX1 genes were assessed by qRT-PCR (n = 6). The experiments in (A-C) were normalized and statistical analysis was performed by one-way ANOVA (Tukey post hoc test). *P < 0.05, **P < 0.005, ***P < 0.001. (D) Protein profiles of CTLA-4, PD-1, TRAIL, TIM-3, CD160 and LAG3 were measured by flow cytometry (n = 3, one representative experiment shown).

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Molecular Medicine

ISSN: 1528-3658