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Figure 4 | Molecular Medicine

Figure 4

From: p38 Mitogen-Activated Protein Kinase/Signal Transducer and Activator of Transcription-3 Pathway Signaling Regulates Expression of Inhibitory Molecules in T Cells Activated by HIV-1-Exposed Dendritic Cells

Figure 4

IL-6 or IL-10 neutralization did not reverse HIV-1-induced T-cell impairment. Mature DCs were pulsed with mock or HIV-1BaL, cultured for ~24 h, washed and cocultured with TN cells for 8 d and restimulated on d 7. Neutralizing anti-IL-6, anti-IL-10, or isotype control mAbs (10 εg/mL) were added and replenished on d 4 and 7 following restimulation. Cocultures were harvested on d 8 to measure T-cell proliferation, gene expression and cytokine secretion. IL-6, IL-10, PDGF, G-CSF, EGF, and VEGF levels were measured in culture supernatants by a Bio-Plex Cytokine Luminex assay. (A) IL-6 and IL-10 cytokine levels in the coculture supernatants. (B) T-cell proliferation was measured for CFSE dilution by flow cytometry in IL-6 and IL-10 neutralized assays (n = 6). (C) PDGF, G-CSF, EGF and VEGF levels in the coculture supernatants. The experiments were normalized and statistical analysis was performed by one-way ANOVA (Tukey post hoc test). *P < 0.05, **P < 0.005.

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Molecular Medicine

ISSN: 1528-3658