MyD88, TLR2 and TLR4 and renal fibrosis. TLR4 (A), TLR2 (B) and MyD88 (C) mRNA were quantified 7 d after surgery and compared to control animals nonsubmitted to UUO. Pelvis proteinuria:creatininuria ratio (D) in all KO and in WT mice 7 d after UUO. FSP-1 staining (E and F) and type-1 collagen mRNA (G) and staining (H and I) at 7 d after surgery in WT, TLR2, TLR4 and MyD88 KO animals. Renal tissue hydroxyproline ratio (J) in WT, TLR2, TLR4 and MyD88 KO animals. Immunohistochemistry is represented by the proportion of the staining area to the total area of the field. qPCR was normalized to HPRT, and the mean of WT control mice was considered 1. Dashed line represents WT animals not submitted to UUO. *p < 0.05, **p < 0.01.