Figure 6

Induction of caspase-independent necrosis by RKA182, FBEG100 and ARS in HepG2 cells. Cells were pretreated (white symbols) or not (black symbols) with the caspase inhibitor Z.VAD.FMK (100 µmol/L, 1 h) and exposed to vehicle (methanol, 0.5%; A), RKA182 (20 µmol/L; B), FBEG100 (60 µmol/L; C), ARS (70 µmol/L; D) or cisplatin (10 µmol/L; E) for a further 24 h. To follow cell death in real-time, annexin V Alexa Fluor 488 (circles) and PI (triangles) staining was measured by live-cell fluorescent imaging at the indicated time points. The total fluorescent intensity of each signal is expressed per cell area. Representative images of annexin and PI signals at the indicated time points, in the absence and presence of Z.VAD.FMK, are presented below the relevant charts.