Figure 4

Changes from baseline in phenotypic markers expressed on CD8 and CD4 T-cells over the 24-wk study period. Changes from baseline in percentage of CD8 T-cells expressing (A) CD28⁺CD27⁺ (early), (B) CD28⁻CD27⁺ (intermediate), (C) CD28⁻CD27⁻ (late) and (D) CD27⁺. (E) Changes from baseline in mean fluorescence intensity (MFI) of CTLA⁻⁴ expressed on CD8 T-cells. Changes from baseline in the percentage of CD4 T-cells expressing (F) CD28⁻CD27⁻ (late) and (G) CD28⁺, and MFI of (H) CD28 and (I) CTLA-4 expressed on CD4⁺ T-cells. Changes from baseline in the percentage of CD4 T-cells expressing (J) CD38⁺HLA-DR⁺ and (K) CD38⁻HLA-DR⁺. Mean changes from baseline estimated by mixed model analysis are plotted with bars above and below representing 95% confidence intervals. Significance from baseline is shown by error bars that do not bisect the x axis, and is marked with *. When significant differences from baseline were lost upon removal of protocol deviators from the analysis, data from all patients is plotted with * shown. Immunizations are indicated by vertical dashed lines. All immunizations were administered at the time point indicated, after blood was drawn for immunological assessments.