Figure 9

HMGB1 diminishes the direct effect of chronic alcohol on induction of macrophage efferocytosis. Peritoneal macrophages were divided into groups as described in the figure. They were treated with culture medium alone (control) or medium containing alcohol (50 mmol/L) for 7 d. Then, macrophages were cocultured with apoptotic thymocytes from 90 min. The coculture medium contained HMGB1 (1 µg/mL) in the group alcohol + HMGB1, whereas coculture medium in other groups was standard DMEM without HMGB1. After treatments, all macrophages were stained and processed for analysis with the flow cytometry as described in the Figure 2 legend. (A) Representative scatter plots in each group. (B) Statistic analysis of FACS assay data. Values are mean ± SEM and represent average of findings from two independent experiments with triplicate samples in each group. *P < 0.05, chronic alcohol versus the control group. #P < 0.05, chronic alcohol versus alcohol + HMGB1.