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Figure 1 | Molecular Medicine

Figure 1

From: Chronic Lymphocytic Leukemia Cells in a Lymph Node Microenvironment Depict Molecular Signature Associated with an Aggressive Disease

Figure 1

Survival, proliferation and upregulation of the NF-κB-associated genes in cocultured CLL cells in vitro with human- or mouse-derived stromal cells. Analyses of cell survival, proliferation and change in expression of key genes in primary CLL cells cocultured with mouse BM-derived OMA-AD and human endothelial-derived HMEC stromal cells at 72 h. The results were analyzed using a Student t test (p < 0.05). (A) Measurement of the proliferation of CLL cells (n = 6) cocultured on stroma using Ki-67 staining. (B) Measurement of the survival of CLL cells (n < 7) cocultured on stroma using annexin V staining. (C) Validation of the upregulation of key genes in CLL cells (n = 6) cocultured on stroma using real-time PCR.

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Molecular Medicine

ISSN: 1528-3658