Figure 5

Correlation of differential gene expression with a prognostic indicator such as BLA and validation of expression of key molecules such as pSyk and CCL3 expression. Gene expression profiles of PB-CLL samples were grouped on the basis of the presence and absence of BLA. (A) Gene expression signature of PB-CLL cells from BLA patients. PB-CLL samples were grouped based on a known prognostic indicator, presence or absence of BLA, and gene expression profiles of these PB-CLL samples were correlated by using significant analysis of microarray analyses. Comparison of expression profiles between PB-CLL with BLA (n = 9) and without BLA (n = 11) are shown. (B) Comparison of the expression of p-SYK in CLL samples from patients with BLA (n = 4) and those without BLA (n = 5). (C) Expression of CCL3 transcripts in BM-CLL with poor chromosomal abnormalities (ChAb) (n = 6) versus good ChAb (n = 11) and LN-CLL with poor ChAb (n = 11) versus good ChAb (n = 3). (D) Relationship between expression of CCL3 transcripts and time to treatment among the additional 40 PB-CLL samples using the Kaplan-Meier log-rank test.