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Figure 5 | Molecular Medicine

Figure 5

From: Signaling of High Mobility Group Box 1 (HMGB1) through Toll-like Receptor 4 in Macrophages Requires CD14

Figure 5

HMGB1 promotes localization of TLR4 coreceptors within the lipid raft. RAW264.7 macrophages were treated with 1 µg/mL HMGB1 for 60 min, and lysates were subjected to sucrose gradient fractionation. Lysates were then drawn off in 12 separate fractions and run on SDS-PAGE before immunoblot (IB) with flotillin-1 to identify raft-enriched fractions. Fractions were then probed for MD2 or CD14. Images shown are representative of at least three independent experiments.

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Molecular Medicine

ISSN: 1528-3658