Figure 7

Suppressive effects of esRAGE and D₆-esRAGE on generation of proinflammatory cytokines including TNF-α, IL-1 and IL-6 in plasma of mice immunized with bovine collagen type II. Treatment of each esRAGE and D₆-esRAGE started to be administered intraperitoneally at a dose of 1 mg/kg per week on d 14 after primary immunization. On d 34, plasma of immunized mice was collected before sacrifice and levels of TNF-α, IL-1 and IL-6 in plasma were measured by ELISA. The elevated TNF-α level in immunized mice was suppressed at the same degree in both esRAGE- and D₆-esRAGE-treated groups. The elevated plasma levels of IL-1 and IL-6 were more suppressed by D₆-esRAGE than esRAGE. Each column with a bar represents the mean ± SEM of six mice. ^*Significantly different from vehicle-treated immunized mice (control) at P < 0.05. ^**Significantly different from vehicle-treated immunized mice (control) at P < 0.01. ND, not detected.