Figure 3

TRPV1^−/− mice show reduced CNS infiltration. (A) Cell counts of inguinal lymph node and spleen prior to and following MOG_35–55 peptide immunization of B6 and TRPV1^−/− mice (n = 9 per group). (B) Quantification of lymphocytes in the CNS by flow cytometry (n > 3 per group, *p < 0.05, **p < 0.01, ***p < 0.001). (C) Representative FACS plot showing distribution of cells within the CNS; CD4⁺ and CD8⁺ cells previously gated on CD3⁺ cells (upper left), CD62L⁺ and CD44⁺ cells previously gated on CD3⁺ cells (lower left), B220⁺ cells (upper right), FoxP3⁺ cells previously gated on CD4⁺ cells (lower right).