Figure 6

TRPV1 increases the permeability of the BBB and BSCB. (A–B) Quantification of Evans Blue in regions of the brain (A, n = 5 per group, *p < 0.05, ***p < 0.001) and the spinal cord (B, n = 5 per group, *p < 0.05, ***p < 0.001) of B6 and TRPV1^−/− mice at indicated time points after induction of EAE, or after injection with MOG_35–55 or pertussis toxin alone. (C) Representative images of brain and spinal cord tissue dissected from B6 and TRPV1^−/− mice 11 d after immunization following Evans Blue injection. (D) Representative images of B6 and TRPV1^−/− whole spinal cord (upper) and sectioned spinal cord (lower) following injection of infrared-labeled IgG 11 d after immunization. (E) Representative images of B6 and TRPV1^−/− whole brain (upper left: coronal and lower left: transverse) and sectioned brain (upper, middle, and lower right) following injection of infrared-labeled IgG 11 d after immunization. (F) Average intensity readings in the lumbar and thoracic regions of the spine following MRI of B6 and TRPV1^−/− mice 11 d after immunization.