Figure 3
Treatment with Ad-BHV1gG-Ig significantly inhibits thioglycollate-induced neutrophil migration in BALB/c mice. Mice treated 4 d previously with Ad-BHV1gG-Ig or control adenovirus were injected IP with sterile thioglycollate. Mice were euthanized 4 or 72 h later and peritoneal cells were analyzed using flow cytometry. (A) Gating strategy and representative FACS plot of peritoneal cells from the mice. Gr-1hi cells (left) were identified as neutrophils whereas macrophages (right) are identified based on their expression of CD11b and F480. Eosinophils are CCR3+. (B) Percentages and numbers of Gr-1hi cells per peritoneal cavity of Ad-BHV1gG-Ig-treated (n = 5), control Ad-BAFF-R-Ig (n = 5), control Ad-LacZ-treated (n = 5) or untreated mice (n = 4) 4 h after thioglycollate treatment. (C) Percentages and numbers of macrophages per peritoneal cavity of Ad-BHV1gG-Ig-treated, Ad-LacZ-treated or untreated mice 72 h after thioglycollate treatment (n = 4 for all groups). Horizontal lines denote the mean value for each group. The P values were compared with Ad-LacZ-treated mice. *P < 0.05, †P < 0.02. Data are representative of three experiments.