Figure 2

CRF₂ actions are key determinants of an inflammatory insult. Representative light micrographs (40x; A, acinar cells, IC, islet cells) of H&E stained pancreas sections from male and female saline-treated WT, caerulein-treated WT, and caerulein-treated Crhr2^−/− mice (A). Graphical representation of histologic damage scores graded by a pathologist is shown. Duplicate sections were scored for necrosis, vacuolization, polymorphonuclear cell infiltration and zymogen granule formation in vehicle-treated WT (Con, white bars), caerulein-treated WT (grey bars) and caerulein-treated Crhr2^−/− (blue bars) mice. Severity scores increased in all histopathologic categories for male Crhr2^−/− mice (*p < 0.05 versus WT) (B). Total score, necrosis and vacuolization increased in female Crhr2^−/− mice (*p< 0.05) (C). Ucn1 treatment (hashed grey and blue bars) significantly decreased all scores for male Crhr2^−/− except PMN infiltrate, which was decreased in male WT mice (**p < 0.05). Ucn1 treatment either worsened histologic damage in female WT and Crhr2^−/− mice or had no effect on variables examined (C). Statistical analyses were performed between same-sex groups by using ANOVA, followed by Bonferroni posttests. Data represent score ± SEM of 12 mice/group.