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Figure 4 | Molecular Medicine

Figure 4

From: Suppression of Coronary Atherosclerosis by Helix B Surface Peptide, a Nonerythropoietic, Tissue-Protective Compound Derived from Erythropoietin

Figure 4

HBSP inhibits apoptosis and activates Akt signaling in coronary artery endothelial cells of WHHLMI rabbits. (A) HBSP reduces apoptosis of coronary artery endothelial cells. Immunostaining of the deparaffinized sections were performed by using anti-CD31 antibody to detect endothelial cells, and an in situ TdT assay was performed. Then the percentage of endothelial cells positive for TdT labeling was determined, as described in Materials and Methods. Data are mean ± SEM; control, n = 7; HBSP n = 8; *P < 0.01. (B) HBSP activates Akt in coronary artery endothelial cells. The deparaffinized sections were incubated with a primary antibody against phospho-Akt or negative control IgG and immunostaining was performed. Then the percentage of endothelial cells positive for phospho-Akt was determined as in (A). Data are mean ± SEM; negative control IgG, n = 3; control, n = 7; HBSP n = 8; *P < 0.01.

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Molecular Medicine

ISSN: 1528-3658